Hyperspectral imaging of moving microscopic sample elements

A system for hyperspectral imaging, comprising:

• a hyperspectral imaging (HSI) device;
• an optical imaging device;
• at least one computing device comprising a processor and memory;
• and
• at least one application executable on the at least one computing device, wherein when executed, the at least one application causes the at least one computing device to at least:
• acquire a series of optical images and corresponding hyperspectral data of a sample, each optical image and its corresponding hyperspectral data acquired simultaneously by the optical imaging device and the HSI device and identified by a time stamp or sequence number;
• compile a movie comprising a series of frames each including one of the series of optical images and a slit line indicating a location of a slit of the HSI device for acquisition of the hyperspectral data;
• and
• analyzing the hyperspectral data corresponding to the optical image in a frame comprising an element of the sample located on the slit line, the analysis generating hyperspectral information about the element.