Method and reagent for testing novel coronavirus

A method for testing a novel coronavirus, the method comprising steps of:

• mixing a specimen sample collected from a subject, and/or a specimen mixed liquid of the specimen sample and a medium, with a specimen treatment liquid containing sodium hydroxide as a main component, to obtain a mixed liquid;
• incubating the mixed liquid;
• adding a master mix containing a reaction liquid, an internal standard substance, a primer, a probe, a reverse transcriptase, and a PCR enzyme to the mixed liquid after the incubation, to obtain a final mixed liquid;
• subjecting the final mixed liquid to a reverse transcription reaction treatment;
• and
• detecting, through use of the probe, DNA amplified by PCR using the DNA generated by the reverse transcription reaction treatment as a template
• wherein an amount of the master mix is 14 μL to 16 μL.