Biliary tract cancer detection kit or device, and detection method

A method for detecting biliary tract cancer in a human subject, comprising:

• measuring an expression level of hsa-miR-16-5p in a blood, serum or plasma sample from the subject;
• comparing the measured expression level of hsa-miR-16-5p to a control expression level for a healthy subject;
• detecting a decreased level of hsa-miR-16-5p in the sample from the subject as compared to the control expression level from the sample from the healthy subject;
• wherein the decreased level of hsa-miR-16-5p indicates that the subject has biliary tract cancer;
• and
• wherein the method further comprises treating the subject for the biliary tract cancer or performing a diagnostic procedure on the subject with the biliary tract cancer;
• wherein the treating comprises surgery, radiotherapy, chemotherapy or a combination thereof;
• and
• wherein the diagnostic procedure comprises a biochemical examination of hepatic dysfunction markers in the sample, detecting a concentration of biliary tract tumor biomarker protein in the sample, or imaging the biliary tract of the human subject.