US 2025/0059599 A1Published application

Detecting apparatus, gene sequencing system, and detecting method

Assigneec/o MGI Tech Co., Ltd.

InventorsYi WEI, Yi HUANG, Heming JIANG, Bin YANG, Xin WEN, Heng HUANG, Mingyou CAO, Qian DENG

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Abstract

The present disclosure provides a detecting apparatus, system, and method, and belongs to the technical field of detection. The detecting apparatus includes a beam splitting device, a first dichroic mirror, an objective lens, a fluorescence guiding device, and an imaging system including a plurality of imaging devices. The beam splitting device receives and separates an incident light beam of a plurality of different wavelengths that is formed by lasers from the optical fiber, and to form a plurality of excitation lasers corresponding to the plurality of different wavelengths of the incident light beam; the first dichroic mirror receives the plurality of excitation lasers exiting from the beam splitting device and transmits them to the objective lens, and receives a plurality of fluorescence and transmits them to the fluorescence guiding device; the objective lens receives the plurality of excitation lasers and focuses them respectively on a plurality of different areas of a sample to excite fluorescence, and transmits the plurality of fluorescence to the first dichroic mirror; and the fluorescence guiding device receives the plurality of fluorescence transmitted via the first dichroic mirror and guides the plurality of fluorescence to the plurality of imaging devices respectively. The present disclosure makes it possible to reduce the power density of the laser on the sample without increasing the detection duration, solving the problem that the two aspects of “high speed” and “high detection quality and long read length” cannot be better compatible in the related arts.

Claims27

Dependent claims

A detecting apparatus, comprising a beam splitting device, a first dichroic mirror, an objective lens, a fluorescence guiding device, and an imaging system comprising a plurality of imaging devices, wherein,

the beam splitting device is configured to receive and separate an incident light beam of a plurality of different wavelengths from an optical fiber, to form a plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam emitted in different emitting directions from the beam splitting device, wherein, each of the excitation light beams has a wavelength in a one-to-one correspondence with the plurality of different wavelengths of the incident light beam;
the first dichroic mirror is positioned to receive the plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam in a one-to-one correspondence and emitted from the beam splitting device, to transmit the plurality of excitation light beams to the objective lens, such that the plurality of excitation light beams are focused respectively on a plurality of different areas of a sample to be detected through the objective lens, to excite a plurality of fluorescence in the plurality of different areas of the sample on which the plurality of excitation light beams are focused respectively, and to receive the plurality of fluorescence excited by the plurality of excitation light beams respectively and transmit the plurality of fluorescence to the fluorescence guiding device;
the objective lens is positioned to receive the plurality of excitation light beams transmitted by the first dichroic mirror, to focus the plurality of excitation light beams on the plurality of different areas of the sample respectively, and to transmit the plurality of fluorescence excited by the plurality of excitation light beams respectively to the first dichroic mirror;
the fluorescence guiding device is positioned to receive the plurality of fluorescence transmitted by the first dichroic mirror, and to guide the plurality of fluorescence to the plurality of imaging devices respectively, such that each fluorescence of the plurality of fluorescence is imaged by one of the plurality of imaging devices corresponding to the fluorescence;
and
each of the plurality of imaging devices is positioned to receive one fluorescence of the plurality of fluorescence guided by the fluorescence guiding device, and to image the fluorescence to obtain fluorescence information corresponding to the fluorescence for detection.

3 more elements

A gene sequencing system, comprising an imaging system for collecting a fluorescence signal on a sequencing chip;

and an optical system located between the imaging system and the sequencing chip, characterized in that the optical system comprises:
a light source configured to emit an incident light beam of a plurality of different wavelengths;
a beam splitting device configured to receive and separate the incident light beam of a plurality different wavelengths from the light source, to form a plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam emitted in different emitting directions from the beam splitting device, wherein, each of the excitation light beams has a wavelength in a one-to-one correspondence with the plurality of different wavelengths of the incident light beam;
a first dichroic mirror configured to receive the plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam in a one-to-one correspondence and emitted from the beam splitting device;
an objective lens arranged between the sequencing chip and the first dichroic mirror and configured to receive the plurality of excitation light beams transmitted by the first dichroic mirror, to focus the plurality of excitation light beams on a plurality of different areas on the sequencing chip to excite a plurality of fluorescence in the plurality of different areas in one-to-one correspondence with the plurality of excitation light beams respectively, and to transmit the plurality of fluorescence to the first dichroic mirror;
and
a fluorescence guiding device configured to receive the plurality of fluorescence transmitted via the first dichroic mirror and to guide the plurality of fluorescence to the imaging system respectively,
wherein the imaging system comprises a plurality of imaging devices corresponding to the plurality of fluorescence in a one-to-one correspondence, each of the plurality of imaging devices being configured to receive one fluorescence, which corresponds to the imaging device, of the plurality of fluorescence guided by the fluorescence guiding device, and to image the fluorescence to obtain fluorescence information corresponding to the fluorescence.

5 more elements

A detecting method, comprising:

emitting, by a light source or a light conduction device, an incident light beam of a plurality of different wavelengths;
receiving and separating, by a beam splitting device, the incident light beam of a plurality of different wavelengths from the light source or the light conduction device, to form a plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam emitted in different emitting directions from the beam splitting device, wherein, each of the excitation light beams has a wavelength in a one-to-one correspondence with the plurality of different wavelengths of the incident light beam;
receiving, by a first dichroic mirror, the plurality of excitation light beams corresponding to the plurality of different wavelengths of the incident light beam in a one-to-one correspondence and emitted from the beam splitting device;
receiving, by an objective lens arranged between a sample to be detected and the first dichroic mirror, the plurality of excitation light beams transmitted by the first dichroic mirror, focusing, by the objective lens, the plurality of excitation light beams on a plurality of different areas on the sample to excite a plurality of fluorescence in the plurality of different areas in one-to-one correspondence with the plurality of excitation light beams respectively, and transmitting, by the objective lens, the plurality of fluorescence to the first dichroic mirror;
and
receiving, by a fluorescence guiding device, the plurality of fluorescence transmitted by the first dichroic mirror, and guiding, by the fluorescence guiding device, the plurality of fluorescence to a plurality of imaging devices corresponding to the plurality of fluorescence in a one-to-one correspondence, such that each fluorescence of the plurality of fluorescence is received and imaged by one of the plurality of imaging devices corresponding to the fluorescence to obtain fluorescence information corresponding to the fluorescence for detection.

3 more elements

Dependencies are inferred from the claim text.