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US 2025/0059596 A1Published application
Methods and compositions for methylation and chemically modifying assisted tailorable ends
AssigneeThe Regents of the University of California
InventorRobert EVANS
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Abstract
The present invention provides for methods and compositions for one-pot Golden Gate cloning, or conventional two-pot restriction enzyme cloning, regardless of internal restriction sites. It uses modified nucleotides during PCR of DNA, or through post-hoc enzymatic modification of already existing DNA, wherein the modification blocks the internal restriction sites while leaving the flanking restriction sites unblocked.
Claims2
A method for one-pot Golden Gate cloning, or conventional two-pot restriction enzyme cloning, regardless of internal restriction sites, comprising:
- using modified nucleotides during PCR of a DNA, or through post-hoc enzymatic modification of already existing DNA, such that the use of the modified nucleotides blocks the internal restriction sites while leaving the flanking restriction sites unblocked.
Dependencies are inferred from the claim text.