Surfactant for analyzing biological material
AssigneeStilla Technologies
InventorSylvain URSUEGUI
Life of this patent
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Abstract
A method for analyzing biological material and a continuous oily phase, which includes a silicone oil and a surfactant, for use in microfluidic devices. The applicant indeed identified a new category of surfactants and an oil/surfactant system which are compatible with molecular biology, improve digital PCR performances and limit sample wasting and dead volume.
Claims15
1-14. (canceled)
A method for analyzing a biological material comprising:
- forming an emulsion which comprises said biological material, involving:
- a continuous oily phase comprising a silicone oil;
- a dispersed aqueous phase;
- a surfactant which is a block copolymer comprising blocks [A], [B] and [C];
- processing said biological material in said emulsion;
- and
- detecting said biological material in said emulsion, wherein:
- block [A] of the surfactant corresponds to a block of formula (I)
- wherein R1 is a C1-C18 alkyl, and m is comprised between 10 and 100;
- block [B] of the surfactant corresponds to a block of formula (II)
- wherein q is comprised between 2 and 18, n is comprised between 1 and 50, and R2 is selected from the group consisting of compounds of
- wherein r, s, t, u, v and w are comprised between 1 and 32 and R3 is selected from the group consisting of —OH, —O—CH3 and —O—CH2CH3;
- block [C] of the surfactant corresponds to a block of formula (VII)
- wherein p is comprised between 5 and 300;
- wherein terminal groups are selected from the group consisting of —H, —CH3 and —Si(CH3)3;
- and wherein the general formula of the surfactant is selected from [A]-[B]—[C], [B]—[C]-[A], [C]-[A]-[B], [B]-[A]-[C], [A]-[C]—[B] and [C]—[B]-[A].
A digital polymerase chain reaction (PCR) mix, comprising a silicone oil and a surfactant as an oily phase in digital PCR, more preferably in digital PCR in droplets, wherein the surfactant is a block copolymer comprising blocks [A], [B] and [C], wherein:
- block [A] of the surfactant corresponds to a block of formula (I)
- wherein R1 is a C1-C18 alkyl, and m is comprised between 10 and 100;
- block [B] of the surfactant corresponds to a block of formula (II)
- wherein q is comprised between 2 and 18, n is comprised between 1 and 50, and R2 is selected from the group consisting of compounds of
- wherein each of r, s, t, u, v and w are comprised between 1 and 32 and R3 is selected from the group consisting of —OH, —O—CH3 and —O—CH2CH3;
- block [C] of the surfactant corresponds to a block of formula (VII)
- wherein p is comprised between 5 and 300;
- wherein terminal groups are selected from the group consisting of —H, —CH3 and —Si(CH3)3;
- and wherein the general formula of the surfactant is selected from [A]-[B]—[C], [B]—[C]-[A], [C]-[A]-[B], [B]-[A]-[C], [A]-[C]—[B] and [C]—[B]-[A].
A continuous oily phase for a microfluidic device comprising a silicone oil and a surfactant, wherein the surfactant is a block copolymer comprising blocks [A], [B] and [C], wherein:
- block [A] of the surfactant corresponds to a block of formula (I)
- wherein R1 is a C1-C18 alkyl, and m is comprised between 10 and 100;
- block [B] of the surfactant corresponds to a block of formula (II)
- wherein q is comprised between 2 and 18, n is comprised between 1 and 50, and R2 is selected from the group consisting of compounds of
- wherein each of r, s, t, u, v and w are comprised between 1 and 32 and R3 is selected from the group consisting of —OH, —O—CH3 and —O—CH2CH3;
- block [C] of the surfactant corresponds to a block of formula (VII)
- wherein p is comprised between 5 and 300;
- wherein terminal groups are selected from the group consisting of —H, —CH3 and —Si(CH3)3;
- wherein the general formula of the surfactant is selected from [A]-[B]—[C], [B]—[C]-[A], [C]-[A]-[B], [B]-[A]-[C], [A]-[C]—[B] and [C]—[B]-[A];
- and wherein the composition comprises at least 0.5% by weight, relative to the total weight of the continuous oily phase, of the surfactant.
A microfluidic device comprising at least one chamber containing a silicone oil and a surfactant, wherein the surfactant is a block copolymer comprising blocks [A], [B] and [C], wherein:
- block [A] of the surfactant corresponds to a block of formula (I)
- wherein R1 is a C1-C18 alkyl, and m is comprised between 10 and 100;
- block [B] of the surfactant corresponds to a block of formula (II)
- wherein q is comprised between 2 and 18, n is comprised between 1 and 50, and R2 is selected from the group consisting of compounds of
- wherein each of r, s, t, u, v and w are comprised between 1 and 32 and R3 is selected from the group consisting of —OH, —O—CH3 and —O—CH2CH3;
- block [C] of the surfactant corresponds to a block of formula (VII)
- wherein p is comprised between 5 and 300;
- wherein terminal groups are selected from the group consisting of —H, —CH3 and —Si(CH3)3;
- wherein the general formula of the surfactant is selected from [A]-[B]—[C], [B]—[C]-[A], [C]-[A]-[B], [B]-[A]-[C], [A]-[C]—[B] and [C]—[B]-[A].
Dependencies are inferred from the claim text.