Improved expression of peptides
AssigneeGelita AG
InventorsStephan Hausmanns, Steffen Oesser, Martin Hahn, Hans-Ulrich Frech, Lars Blank, Jochen BÜCHS, Andrea Germer, David Wollborn
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Abstract
The present invention relates to fungal cells, in particular methylotrophic fungal cells, for use in improved production of recombinant peptides of interest and processes for producing recombinant peptides of interest.
Claims21
A fungal cell, which fungal cell comprises at least one copy of an exogenous nucleic acid sequence of interest stably integrated in the yur1 gene of the fungal cell, which exogenous nucleic acid sequence of interest encodes a recombinant peptide of interest.
A fungal cell, which is a Komagataella phaffii cell, as deposited with the DSMZ under accession numbers:
- Komagataella phaffii 45I-1 (DSM 33955, deposited on 28 Jul. 2021), Komagataella phaffii 45I-2 (DSM 33956, deposited on 28 Jul. 2021), Komagataella phaffii 45I-3 (DSM 33957, deposited on 28 Jul. 2021), a further derivate of Komagataella phaffii 45I-1, Komagataella phaffii 45I-4 (DSM 33958, deposited on 28 Jul. 2021) or a derivate of Komagataella phaffii 45I-4.
An expression vector comprising an expression cassette, which expression cassette comprises at least one nucleic acid sequence of interest, in particular encoding a collagen peptide,
- and at least one nucleic acid sequence of at least 10, preferably at least 25, preferably at least 50, preferably at least 100, preferably at least 150, preferably all, nucleotides of the yur1 gen, preferably as identified in SEQ ID No. 13.
(canceled)
Dependencies are inferred from the claim text.