US 2025/0059577 A1 · REVERSE TRANSCRIPTASE HAVING EXCELLENT THERMAL STABILITY

Abstract

Provided is a novel reverse transcriptase with excellent thermal stability. A reverse transcriptase comprising the following amino acid sequence: (a) an amino acid sequence having at least 75% identity with the amino acid sequence represented by SEQ ID NO: 2 or 3; or (b) an amino acid sequence having deletion, substitution, insertion, or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO: 2 or 3.

Claims19

Dependent claims

A reverse transcriptase comprising the following amino acid sequence:

(a) an amino acid sequence having at least 75% identity with the amino acid sequence represented by SEQ ID NO:
2 or 3;
or
(b) an amino acid sequence having deletion, substitution, insertion, or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO:
2 or 3.

2 more elements

A reverse transcriptase

which has at least 80% identity with the amino acid sequence represented by SEQ ID NO:
1, and having at least one amino acid residue selected from the group consisting of the following (1) to (43):
(1) an amino acid at a position corresponding to position 5 is L,
(2) an amino acid at a position corresponding to position 47 is P,
(3) an amino acid at a position corresponding to position 51 is V,
(4) an amino acid at a position corresponding to position 52 is E,
(5) an amino acid at a position corresponding to position 62 is V,
(6) an amino acid at a position corresponding to position 85 is L,
(7) an amino acid at a position corresponding to position 87 is V,
(8) an amino acid at a position corresponding to position 89 is R,
(9) an amino acid at a position corresponding to position 94 is A,
(10) an amino acid at a position corresponding to position 144 is R,
(11) an amino acid at a position corresponding to position 160 is P,
(12) an amino acid at a position corresponding to position 219 is T,
(13) an amino acid at a position corresponding to position 232 is D,
(14) an amino acid at a position corresponding to position 233 is T,
(15) an amino acid at a position corresponding to position 235 is E,
(16) an amino acid at a position corresponding to position 266 is E,
(17) an amino acid at a position corresponding to position 276 is G,
(18) an amino acid at a position corresponding to position 338 is T,
(19) an amino acid at a position corresponding to position 350 is K,
(20) an amino acid at a position corresponding to position 354 is S,
(21) an amino acid at a position corresponding to position 377 is I,
(22) an amino acid at a position corresponding to position 414 is I,
(23) an amino acid at a position corresponding to position 419 is L,
(24) an amino acid at a position corresponding to position 425 is D,
(25) an amino acid at a position corresponding to position 429 is L,
(26) an amino acid at a position corresponding to position 463 is S,
(27) an amino acid at a position corresponding to position 476 is P,
(28) an amino acid at a position corresponding to position 477 is A,
(29) an amino acid at a position corresponding to position 495 is D,
(30) an amino acid at a position corresponding to position 519 is E,
(31) an amino acid at a position corresponding to position 523 is F,
(32) an amino acid at a position corresponding to position 532 is D,
(33) an amino acid at a position corresponding to position 542 is V,
(34) an amino acid at a position corresponding to position 569 is K,
(35) an amino acid at a position corresponding to position 573 is L,
(36) an amino acid at a position corresponding to position 578 is R,
(37) an amino acid at a position corresponding to position 581 is I,
(38) an amino acid at a position corresponding to position 597 is A,
(39) an amino acid at a position corresponding to position 600 is Q,
(40) an amino acid at a position corresponding to position 632 is A,
(41) an amino acid at a position corresponding to position 646 is V,
(42) an amino acid at a position corresponding to position 658 is K, and
(43) an amino acid at a position corresponding to position 662 is L, or
which has at least 80% identity with the amino acid sequence represented by SEO ID NO:
1, and has at least one amino acid residue selected from the group consisting of the following (2), (4), (6), (8), (9), (11), (12), (13), (15), (16), (19), (21), (24), (26), (27), (29), (33), (34), (38), (39), and (41):
(2) an amino acid at a position corresponding to position 47 is P,
(4) an amino acid at a position corresponding to position 52 is E,
(6) an amino acid at a position corresponding to position 85 is L,
(8) an amino acid at a position corresponding to position 89 is R,
(9) an amino acid at a position corresponding to position 94 is A,
(11) an amino acid at a position corresponding to position 160 is P,
(12) an amino acid at a position corresponding to position 219 is T,
(13) an amino acid at a position corresponding to position 232 is D,
(15) an amino acid at a position corresponding to position 235 is E,
(16) an amino acid at a position corresponding to position 266 is E,
(19) an amino acid at a position corresponding to position 350 is K,
(21) an amino acid at a position corresponding to position 377 is L,
(24) an amino acid at a position corresponding to position 425 is D,
(26) an amino acid at a position corresponding to position 463 is S,
(27) an amino acid at a position corresponding to position 476 is P,
(29) an amino acid at a position corresponding to position 495 is D,
(33) an amino acid at a position corresponding to position 542 is V,
(34) an amino acid at a position corresponding to position 569 is K,
(38) an amino acid at a position corresponding to position 597 is A,
(39) an amino acid at a position corresponding to position 600 is Q, and
(41) an amino acid at a position corresponding to position 646 is V, or
which has at least 80% identity with the amino acid sequence represented by SEO ID NO:
1, and has at least one amino acid residue selected from the group consisting of the following (2), (5), (10), (14), (20), (25), (27), (29), (33), and (43):
(2) an amino acid at a position corresponding to position 47 is P,
(5) an amino acid at a position corresponding to position 62 is V,
(10) an amino acid at a position corresponding to position 144 is R,
(14) an amino acid at a position corresponding to position 233 is T,
(20) an amino acid at a position corresponding to position 354 is S,
(25) an amino acid at a position corresponding to position 429 is L,
(27) an amino acid at a position corresponding to position 476 is P,
(29) an amino acid at a position corresponding to position 495 is D,
(33) an amino acid at a position corresponding to position 542 is V, and
(43) an amino acid at a position corresponding to position 662 is L.

77 more elements

Dependencies are inferred from the claim text.

Facts

Application

18/721,137Filed 14 Dec 2022

Priority

JP 2021-20552517 Dec 2021

Published

20 Feb 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 5/10
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: cells…
C12P 19/34
Nucleotides: polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12N 9/1276
Nucleotidyltransferases: rNA-directed DNA polymerase , i.e. reverse transcriptase or telomerase
C12N 15/1096
Processes for the isolation, preparation or purification of DNA or RNA: cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Abstract

Claims19

Dependent claims

A reverse transcriptase comprising the following amino acid sequence:

(a) an amino acid sequence having at least 75% identity with the amino acid sequence represented by SEQ ID NO:
2 or 3;
or
(b) an amino acid sequence having deletion, substitution, insertion, or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO:
2 or 3.

2 more elements

A reverse transcriptase

which has at least 80% identity with the amino acid sequence represented by SEQ ID NO:
1, and having at least one amino acid residue selected from the group consisting of the following (1) to (43):
(1) an amino acid at a position corresponding to position 5 is L,
(2) an amino acid at a position corresponding to position 47 is P,
(3) an amino acid at a position corresponding to position 51 is V,
(4) an amino acid at a position corresponding to position 52 is E,
(5) an amino acid at a position corresponding to position 62 is V,
(6) an amino acid at a position corresponding to position 85 is L,
(7) an amino acid at a position corresponding to position 87 is V,
(8) an amino acid at a position corresponding to position 89 is R,
(9) an amino acid at a position corresponding to position 94 is A,
(10) an amino acid at a position corresponding to position 144 is R,
(11) an amino acid at a position corresponding to position 160 is P,
(12) an amino acid at a position corresponding to position 219 is T,
(13) an amino acid at a position corresponding to position 232 is D,
(14) an amino acid at a position corresponding to position 233 is T,
(15) an amino acid at a position corresponding to position 235 is E,
(16) an amino acid at a position corresponding to position 266 is E,
(17) an amino acid at a position corresponding to position 276 is G,
(18) an amino acid at a position corresponding to position 338 is T,
(19) an amino acid at a position corresponding to position 350 is K,
(20) an amino acid at a position corresponding to position 354 is S,
(21) an amino acid at a position corresponding to position 377 is I,
(22) an amino acid at a position corresponding to position 414 is I,
(23) an amino acid at a position corresponding to position 419 is L,
(24) an amino acid at a position corresponding to position 425 is D,
(25) an amino acid at a position corresponding to position 429 is L,
(26) an amino acid at a position corresponding to position 463 is S,
(27) an amino acid at a position corresponding to position 476 is P,
(28) an amino acid at a position corresponding to position 477 is A,
(29) an amino acid at a position corresponding to position 495 is D,
(30) an amino acid at a position corresponding to position 519 is E,
(31) an amino acid at a position corresponding to position 523 is F,
(32) an amino acid at a position corresponding to position 532 is D,
(33) an amino acid at a position corresponding to position 542 is V,
(34) an amino acid at a position corresponding to position 569 is K,
(35) an amino acid at a position corresponding to position 573 is L,
(36) an amino acid at a position corresponding to position 578 is R,
(37) an amino acid at a position corresponding to position 581 is I,
(38) an amino acid at a position corresponding to position 597 is A,
(39) an amino acid at a position corresponding to position 600 is Q,
(40) an amino acid at a position corresponding to position 632 is A,
(41) an amino acid at a position corresponding to position 646 is V,
(42) an amino acid at a position corresponding to position 658 is K, and
(43) an amino acid at a position corresponding to position 662 is L, or
which has at least 80% identity with the amino acid sequence represented by SEO ID NO:
1, and has at least one amino acid residue selected from the group consisting of the following (2), (4), (6), (8), (9), (11), (12), (13), (15), (16), (19), (21), (24), (26), (27), (29), (33), (34), (38), (39), and (41):
(2) an amino acid at a position corresponding to position 47 is P,
(4) an amino acid at a position corresponding to position 52 is E,
(6) an amino acid at a position corresponding to position 85 is L,
(8) an amino acid at a position corresponding to position 89 is R,
(9) an amino acid at a position corresponding to position 94 is A,
(11) an amino acid at a position corresponding to position 160 is P,
(12) an amino acid at a position corresponding to position 219 is T,
(13) an amino acid at a position corresponding to position 232 is D,
(15) an amino acid at a position corresponding to position 235 is E,
(16) an amino acid at a position corresponding to position 266 is E,
(19) an amino acid at a position corresponding to position 350 is K,
(21) an amino acid at a position corresponding to position 377 is L,
(24) an amino acid at a position corresponding to position 425 is D,
(26) an amino acid at a position corresponding to position 463 is S,
(27) an amino acid at a position corresponding to position 476 is P,
(29) an amino acid at a position corresponding to position 495 is D,
(33) an amino acid at a position corresponding to position 542 is V,
(34) an amino acid at a position corresponding to position 569 is K,
(38) an amino acid at a position corresponding to position 597 is A,
(39) an amino acid at a position corresponding to position 600 is Q, and
(41) an amino acid at a position corresponding to position 646 is V, or
which has at least 80% identity with the amino acid sequence represented by SEO ID NO:
1, and has at least one amino acid residue selected from the group consisting of the following (2), (5), (10), (14), (20), (25), (27), (29), (33), and (43):
(2) an amino acid at a position corresponding to position 47 is P,
(5) an amino acid at a position corresponding to position 62 is V,
(10) an amino acid at a position corresponding to position 144 is R,
(14) an amino acid at a position corresponding to position 233 is T,
(20) an amino acid at a position corresponding to position 354 is S,
(25) an amino acid at a position corresponding to position 429 is L,
(27) an amino acid at a position corresponding to position 476 is P,
(29) an amino acid at a position corresponding to position 495 is D,
(33) an amino acid at a position corresponding to position 542 is V, and
(43) an amino acid at a position corresponding to position 662 is L.

77 more elements

Dependencies are inferred from the claim text.

Reverse transcriptase having excellent thermal stability

Life of this patent

Abstract

Claims19

Reverse transcriptase having excellent thermal stability

Life of this patent

Abstract

Claims19