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US 2025/0059568 A1Published application
Class ii, type v crispr systems
AssigneeMetagenomi, Inc.
InventorsBrian THOMAS, Christopher BROWN, Audra DEVOTO, Cristina BUTTERFIELD, Lisa ALEXANDER, Daniela S.A. GOLTSMAN, Justine ALBERS, Alan BROOKS, Greg COST, Morayma TEMOCHE-DIAZ, Cindy CASTELLE, Rebecca LAMOTHE
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Abstract
Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.
Claims21
-164. (canceled)
A method of disrupting a TRAC locus in a cell, said method comprising introducing to said cell:
- (a) an endonuclease or a polynucleotide encoding said endonuclease, wherein said endonuclease is configured to be selective for a protospacer adjacent motif (PAM) sequence comprising 5′-YYn-3′ (SEQ ID NO:3871), and wherein said endonuclease comprises a WED II domain and a PAM-interacting domain having at least 80% sequence identity to a WED II domain and a PAM-interacting domain, respectively, of the amino acid sequence of SEQ ID NO:
- 215;
- and
- (b) an engineered guide ribonucleic acid or a polynucleotide encoding said engineered guide ribonucleic acid, wherein said engineered guide ribonucleic acid is configured to form a complex with said endonuclease, wherein said engineered guide ribonucleic acid comprises a spacer sequence configured to hybridize to a region of said TRAC locus, and wherein said engineered guide ribonucleic acid comprises a nucleotide sequence having at least 80% sequence identity to any one of SEQ ID NOs:
- 4378, 4394, 4404, 4370-4377, 4379-4393, 4395-4403, or 4405-4423.
2 more elements
Dependencies are inferred from the claim text.