Payload delivery across cell membranes using continuous flow fluidic system

A device comprising a substrate containing fluidic channels, the substrate comprising:

• a first fluidic channel including a first inlet configured to receive a suspension including a population of cells;
• a second fluidic channel including a second inlet configured to receive a delivery solution including a delivery payload;
• a mixing channel that connects the first fluidic channel and the second fluidic channel such that the first fluidic channel and the second fluidic channel are in fluidic communication, the mixing channel configured to mix the suspension and the delivery solution;
• a process chamber connected to the mixing channel configured to retain the mixture of the suspension and the delivery solution for a period of time for exposing the population of cells to the delivery solution;
• a third fluidic channel including a third inlet configured to receive a stop solution;
• an
• a dilution channel connected to the process chamber and the third fluidic channel, the dilution channel configured to combine and output fluid of the process chamber with the stop solution supplied from the third fluidic channel.