Optogenetic circuits for controlling chemical and protein production in escherichia coli
AssigneeThe Trustees of Princeton University
InventorsJosé L. Avalos, Makoto A. Lalwani, César Carrasco-López
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Abstract
Disclosed herein are optogenetic circuits for the bacterium Escherichia coli that induce gene expression in darkness and repress it under blue light. Applying them to metabolic engineering improves chemical production compared to chemically induced controls in light-controlled fermentations. More particularly, these circuits can be used to control protein production with light. The system and method use light as a suitable alternative to chemical induction for microbial production of chemicals and proteins.
Claims11
A method for controlling growth and chemical production with light, comprising:
- providing at least one bacterial cell;
- controlling the growth of the at least one bacterial cell and production of a target chemical by varying a light condition, where the at least one bacterial cell is grown under a first light condition and the target chemical is produced under a second light condition.
Dependencies are inferred from the claim text.