COMPOSITIONS AND METHODS OF tRNA SILENCING
OwnerSandhya Pande, Anupama Lakshmanan +5inventors
InventorsSandhya Pande, Anupama Lakshmanan, Lauriel Earley, Stephen Michael Burleigh, Collin Hauskins, Adrian Wrangham Briggs, Kevin Stein
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Abstract
Provided are compositions and methods for silencing suppressor tRNAs. Further provided are compositions and methods for silencing suppressor tRNAs during AAV production for producing virions comprising sequences coding for suppressor tRNAs.
Claims95
A polynucleotide comprising, from 5′ to 3′:
- a 5′ AAV inverted terminal repeat (ITR) sequence;
- a polynucleotide sequence comprising one or more conditionally repressible suppressor tRNA coding sequence(s);
- and
- a 3′ AAV inverted terminal repeat (ITR) sequence.
The polynucleotide of claim 2 or claim 3, wherein the sequence encoding the repressor element is upstream of the sequence encoding the suppressor tRNA.
The polynucleotide of claim 16, wherein the spacer sequence is TC.
A composition comprising one or more stop codon engineered AAV nucleotide sequence(s).
Dependencies are inferred from the claim text.