Methods of optimizing gene expression in plant
AssigneePlantarc Bio Ltd.
InventorsDror SHALITIN, Noam GRIMBERG, Daniel BECHAR, Tal COHEN
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Abstract
A method for in-planta high throughput evaluation of endogenous genetic regulatory elements of a crop plant, obtaining a nucleic acid encoding a native regulatory element of a trait-related gene, introducing one or more genetic changes in each of a plurality of copies of the native regulatory element, introducing the genetic library into model plants, utilizing high throughput transformation, screening the transformed model plants, and identifying the one or more genetic changes in the altered regulatory element of the selected model plant.
Claims21
-27. (canceled)
A method for in-planta high throughput evaluation of endogenous genetic regulatory elements of a crop plant, the method comprising the steps of:
- a. obtaining a nucleic acid encoding a native regulatory element of a trait-related gene, coupling it to a coding sequence of a reporter gene;
- wherein the regulatory element is of the crop plant;
- b. introducing one or more genetic changes in each of a plurality of copies of the native regulatory element, thereby obtaining a genetic library comprising a large collection of altered regulatory elements;
- c. introducing the genetic library into model plants, utilizing high throughput transformation, such that on average each model plant receives a single altered regulatory element;
- d. screening the transformed model plants, wherein the screening comprises selecting a model plant having a desired change in a expression level/phenotype of the reporter gene;
- e. identifying the one or more genetic changes in the altered regulatory element of the selected model plant.
A gene edited crop plant or crop plant cell comprising a ppo1 promoter genetically modified to include one or more mutations in one or more positions corresponding to any one or more of positions:
- −1229 A, −1105 T, −668 A, −481 T, −189 A, −70 T, and −61 T of a soybean promoter having a nucleic acid sequence as set forth in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.