Ligands and methods of making ligands for affinity capture on a surface of mrna in a solution

A method for modifying a surface for purification of mRNA, comprising:

• providing a substrate having a surface;
• and
• grafting a plurality of ligands on the surface to form a ligand layer, wherein the ligand layer includes:
• a linker bound to the surface;
• and
• one or more oligomer constructs bound to the linker,
• wherein the oligomer constructs include between 5% and about 10% guanine and about 90% to about 95% thymine.

A modified substrate for purification of mRNA, comprising:

• a surface;
• and
• a ligand layer on the surface,
• wherein the ligand layer includes a plurality of ligands including:
• a linker construct bound to the surface, the linker construct including an acrylate group, an azide group, or combinations thereof;
• one or more spacer constructs bound to the linker construct, wherein the one or more spacer constructs includes a polyethyleneglycol (PEG) group, a hydrocarbyl group, or combinations thereof;
• and
• one or more oligomer constructs bound to at least one spacer construct,
• wherein the oligomer constructs include between 5% and about 10% guanine and about 90% to about 95% thymine, and between about 20 and about 60 nucleotides including at least 15 thymines and at least 1 guanine.