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AssigneeDsm IP Assets B.V.
InventorsViktor Marius BOER, Priscilla ZWARTJENS, Johannes Gustaaf Ernst VAN LEEUWEN
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The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
A variant polypeptide having geranylgeranyl pyrophosphate synthase activity, wherein the variant polypeptide comprises an amino acid sequence having at least 80% sequence identity with the amino acid sequence of SEQ ID NO:17 and which comprises a substitution of an amino acid residue corresponding to any of amino acids at positions 89, 97, or 225, with a different amino acid.
Dependencies are inferred from the claim text.