Device and method for creating organoids, and cell culture

A device for producing an arrangement for creating organoids (06) each simulating a biological organ having an organ development state;

• wherein the device comprises the following components:
• a substrate (01) which has a plurality of cavities (02) which are each designed, in their size and form, to receive the biological organ to be simulated having the organ development state to be achieved, wherein the cavities (02) each have the form of a recess, wherein the recesses each have a length and a width, wherein the length is greater than the width, and wherein the width and the length of the recess are adapted to the microfilaments (03) to be received, in such a way that the microfilaments (03) each have a length which is greater than the width and smaller than the length of the recesses;
• a reservoir (17) for storing a transport liquid containing a plurality of microfilaments (03);
• a flushing device (07) with a substrate holder (11) for holding the substrate (01), wherein the flushing device (07) comprises an inlet (12) for the transport liquid containing the microfilaments (03), which inlet opens out into a volume above the substrate holder (11);
• and
• a pump (16) for conveying the transport liquid containing the microfilaments (03) from the reservoir (17) into the inlet (12) of the flushing device (07), wherein the transport liquid is pumped through pores (04) in the cavities (02) in the substrate (01), so that the individual microfilaments (03) enter the cavities (02) and remain there because of their size, while the transport liquid flows through the pores (04) of the substrate.