Devices and methods for preparing in vitro models of neuromuscular junctions

A cell culture device for preparing an in vitro model of a neuromuscular junction, the cell culture device comprising:

• a cell culture layer comprising:
• a neuronal cells channel configured for receiving neuronal cells therein and comprising:
• a first portion having a first portion cross-section;
• and
• a second portion having a second portion cross-section smaller than the first portion cross-section and provided downstream of the first portion;
• and
• a neuromuscular junction chamber in fluid communication with the neuronal cells channel and configured to receive muscle cells therein to enable co-culture of the neuronal cells with the muscle cells and form the neuromuscular junction, the neuromuscular junction chamber comprising:
• first and second pillars provided in a spaced-apart relationship on either side of the neuronal cells channel for culturing the muscle cells therebetween.

A cell culture device for preparing an in vitro model of a neuromuscular junction, the cell culture device comprising:

• a cell culture layer comprising:
• a neuronal cells channel comprising microchannels configured to receive axons from neuronal cells therein for directing axonal growth;
• and
• a neuromuscular junction chamber in fluid communication with the neuronal cells channel and configured to receive muscle cells therein to enable co-culture of the neuronal cells with the muscle cells and form the neuromuscular junction, the neuromuscular junction chamber comprising:
• first and second pillars provided in a spaced-apart relationship on either side of the neuronal cells channel for culturing the muscle cells therebetween.