Artificial cell death polypeptide for chimeric antigen receptor and uses thereof

An iPSC, a natural killer (NK) cell or a T cell comprising:

• (i) a first exogenous polynucleotide encoding a chimeric antigen receptor (CAR);
• (ii) a second exogenous polynucleotide encoding a truncated epithelial growth factor (tEGFR) variant having the amino acid sequence of SEQ ID NO:
• 71, an autoprotease peptide having the amino acid sequence of SEQ ID NO:
• 73, and interleukin 15 (IL-15) having the amino acid sequence of SEQ ID NO:
• 72;
• and
• (iii) optionally, a third exogenous polynucleotide encoding a human leukocyte antigen E (HLA-E) having the amino acid sequence of SEQ ID NO:
• 66;
• wherein the first, second and third exogenous polynucleotides are integrated at loci of AAVS1, CIITA and B2M genes, to thereby delete or reduce expression of CIITA and B2M.