Freeze-dried viral combination vaccine compositions and process for preparation thereof

A kit comprising:

• a) a first container containing a lyophilized (freeze-dried) viral combination vaccine composition comprising:
• a virus consisting of any one or more of live attenuated measles virus present at a dose of not less than 3 log10 CCID50 per 0.5 ml, live attenuated rubella virus present at a dose of not less than 3 log10 CCID50 per 0.5 ml or live attenuated Coronavirus present at a dose of not less than 3 log10 PFU per 0.5 ml;
• a carbohydrate consisting of sorbitol present at a concentration of 1 to 10% (w/v);
• an amino acid consisting of any one or more tricine present at a concentration of 0.1% to 2% (w/v), L-histidine present at a concentration of 0.1% to 2% (w/v), L-alanine present at a concentration of 0.01% to 1% (w/v) or L-arginine hydrochloride present at a concentration of 0.1% to 5% (w/v);
• and a hydrolyzed protein consisting of any one or more of gelatin present at a concentration of 0.1% to 5% (w/v) or lactalbumin hydrolysate present at a concentration of 0.1% to 2% (w/v);
• and
• b) a second container containing an aqueous solution selected from any one or more of saline or water for injection (WFI) for the reconstitution of the lyophilized (freeze-dried) vaccine composition.