Adenoviral vector system for gene delivery
OwnerSuresh Kumar Mittalinventor
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Abstract
Disclosed herein a unique cell line system to generate a novel bovine adenovirus vector that provides more gene insertion capabilities and better immunogenicity for inserted antigens. The unique cell line is used for generating and growing of the new adenovirus vectors for gene delivery or recombinant vaccine production.
Claims20
A method of generating immunogenicity against a virus in a subject, comprising administering to said subject an effective amount of BAdV3 vaccine expressing an antigen of said virus antigen, wherein said BAdV3 vaccine is produced in a bovine cell line co-transfected with:
- a. a human adenovirus (HAdV) E1 region;
- b. a bovine adenovirus E1BL gene;
- and
- c. a bovine adenovirus type 3 (BAdV3) vector with E1A, E1BS, E1BL and E3 regions replaced by a gene of said virus antigen.
A vaccine production system vaccine production system comprising a bovine cell line co-transfected with
- a. human adenovirus (HAdV) E1 region;
- b. a bovine adenovirus E1BL gene;
- and
- c. a bovine adenovirus type 3 (BAdV3) vector with E1A, E1BS, E1BL and E3 regions replaced by a foreign antigenic gene.
A method of generating immunogenicity against H5N1 influenza virus hemagglutinin (HA) antigen in a subject, comprising administering to said subject an effective amount of BAdV3 vaccine expressing H5HA, wherein said BAdV3 vaccine expressing H5HA is produced in a bovine cell line co-transfected with:
- a. a human adenovirus (HAdV) E1 region;
- b. a bovine adenovirus E1BL gene;
- and
- c. a bovine adenovirus type 3 (BAdV3) vector with E1A, E1BS, E1BL and E3 regions replaced by H5HA gene of H5N1 influenza virus.
Dependencies are inferred from the claim text.