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US 2025/0057932 A1Published application
Vaccines against infectious diseases caused by positive stranded rna viruses
AssigneeMedigen, Inc.
InventorsPeter Pushko, Irina Tretyakova
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Abstract
Compositions for protecting subjects from diseases caused by (+)SS RNA virus are described herein. The compositions include (i) a vector containing a DNA encoding a RNA molecule of an infectious (+)SS RNA virus operably linked to a eukaryotic RNA polymerase promoter and a carrier; or (ii) (+)SS RNA viruses obtained from eukaryotic cells transfected with the vector of (i) and a carrier.
Claims17
A method of improving yield of a DNA encoding a RNA molecule operatively linked to a promoter suitable for expression DNA in a eukaryotic cell,
- wherein the method comprises:
- introducing three or more introns into the DNA, and wherein at least one intron is in a region encoding a non-structural protein and at least one intron is in a region encoding a structural protein;
- transfecting the DNA into a host cell;
- and
- isolating the DNA from the host cell;
- wherein the yield of the DNA is increased as compared to a DNA encoding a RNA molecule comprising less than three introns;
- and
- wherein the RNA molecule encodes an infectious positive single stranded ((+)SS) RNA virus.
5 more elements
Dependencies are inferred from the claim text.