System and method for quantitative phase imaging for multi-parametric screening of tumor therapy response

A method for determining treatment response parameters with a microscope imaging system, the method comprising:

• receiving, by an electronic controller, a plurality of images of a plurality of therapy treatment samples over a period of time from a camera;
• determining, via the electronic controller, a cell mass for each therapy treatment sample of the plurality of therapy treatment samples based on each image of the plurality of images;
• tracking, via the electronic controller, the cell mass over the period of time for each therapy treatment sample of the plurality of therapy treatment samples;
• and
• determining, via the electronic controller, a plurality of response parameters for each therapy treatment sample of the plurality of therapy treatment samples based on the cell mass over the period of time.

A quantitative phase imaging microscope comprising:

• a camera configured to capture images of a plurality of therapy treatment samples over a period of time;
• and
• an electronic controller communicatively coupled to the quantitative phase imaging microscope, the electronic controller configured to:
• receive a plurality of images of the plurality of therapy treatment samples over the period of time from the camera;
• determine a cell mass for each therapy treatment sample of the plurality of therapy treatment samples based on each image of the plurality of images;
• track the cell mass over the period of time for each therapy treatment sample of the plurality of therapy treatment samples;
• and
• determine a plurality of response parameters for each therapy treatment sample of the plurality of therapy treatment samples based on the cell mass over the period of time.