Engineered particles as red blood cell mimics and compositions containing same for hematology
AssigneeSlingshot Biosciences, Inc.
InventorsDaixuan ZHANG, Jeffrey KIM, Keunho AHN
Life of this patent
Estimating the term…
Abstract
Provided herein are red blood cell control compositions comprising one or more populations of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter; and a population of hemoglobin molecules or a population of dye molecules that have substantially similar absorbance as hemoglobin; wherein (i) and/or (ii) are present in an amount that corresponds to a normal blood sample or disease state, or (i) and (ii) are present at a ratio that corresponds to a normal blood sample or disease state.
Claims28
A synthetic whole blood sample control, comprising:
- (i) a population of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter;
- (ii) a population of hemoglobin molecules and/or a population of dye molecules that has substantially similar absorbance as hemoglobin;
- and
- (iii) a population of non-lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human white blood cell of average diameter.
A method of calibrating a hematology analyzer for analysis of a target whole blood sample comprising red blood cells and white blood cells, the method comprising:
- (a) inserting into the hematology analyzer a synthetic whole blood sample control, wherein the synthetic whole blood sample control comprises:
- (i) a population of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter;
- (ii) a population of hemoglobin molecules and/or a population of dye molecules that has substantially similar absorbance as hemoglobin;
- and
- (iii) a population of non-lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human white blood cell of average diameter,
- (b) measuring the impedance of the population of lysable hydrogel particles and the population of non-lysable hydrogel particles using the hematology analyzer;
- (c) calibrating the hematology analyzer for analysis of the red blood cells based on the measured impedance of the lysable hydrogel particles, and calibrating the hematology analyzer for analysis of the white blood cells based on the measured impedance of the population of non-lysable hydrogel particles.
A method of evaluating a target whole blood sample comprising red blood cells and white blood cells, the method comprising:
- (a) inserting into a hematology analyzer a synthetic whole blood control, wherein the synthetic whole blood control comprises:
- (i) a population of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter;
- (ii) a population of hemoglobin molecules and/or a population of dye molecules that has substantially similar absorbance as hemoglobin;
- and
- (iii) a population of non-lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human white blood cell of average diameter,
- (b) measuring the impedance of the population of the lysable hydrogel particles and the impedance of the population of the non-lysable hydrogel particles,
- (c) measuring the impedance of the red blood cells and the white blood cells in the target whole blood sample,
- (d) comparing the impedance of the red blood cells in the target whole blood sample with the impedance of the population of the lysable hydrogel particles in the synthetic whole blood control, and
- comparing the impedance of the white blood cells in the target whole blood sample with the impedance of the population of the non-lysable hydrogel particles in the synthetic whole blood control.
A method of evaluating a target whole blood sample comprising red blood cells and white blood cells, the method comprising:
- (a) inserting into a hematology analyzer a synthetic whole blood control, wherein the synthetic whole blood control comprises:
- (i) a population of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter;
- (ii) a population of hemoglobin molecules and/or a population of dye molecules that has substantially similar absorbance as hemoglobin;
- and
- (iii) a population of non-lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human white blood cell of average diameter,
- (b) measuring the impedance of the population of lysable hydrogel particles and comparing the impedance of the red blood cells in the target whole blood sample with the impedance of the population of lysable hydrogel particles in the synthetic whole blood control,
- (c) bringing the synthetic whole blood control in contact with a lysis buffer, wherein the population of the lysable hydrogel particles comprise a bifunctional monomer comprising a bond that is capable of being lysed by the lysis buffer, and
- (d) measuring the impedance of the population of the non-lysable hydrogel particles and comparing the impedance of the white blood cells in the target whole blood sample with the impedance of the population of non-lysable hydrogel particles in the synthetic whole blood control.
Dependencies are inferred from the claim text.