Glx-derived molecule detection

An ex vivo method of diagnosing and/or prognosticating at least one immune-mediated inflammatory disease (IMID) in a subject, said method comprising:

• a. performing an in vitro measurement of the level of one or more biomarkers selected from the group consisting of glycosaminoglycans (GAGs) and proteoglycans (PGs) of the glycocalyx in a first biological sample from the subject;
• b. optionally, performing an in vitro measurement of the level of the one or more biomarkers in a second or additional biological samples from the subject obtained at a later time point than said first biological sample;
• c. comparing the level of said one or more biomarkers to the level of one or more corresponding references;
• and
• d. using the measured value to evaluate the IMID state of the subject.