Single particle analysis method for viral nucleic acid vector

A method for analyzing one or more parameters of a viral nucleic acid vector, comprising:

• step (1):
• preparing a fluorescence labeling reagent that can specifically bind to the viral nucleic acid vector and mixing the fluorescence labeling reagent with a sample comprising the viral nucleic acid vector;
• the fluorescence labeling reagent comprising a nucleic acid probe, a nucleic acid stain, and at least one selected from a lipid membrane dye and a fluorophore-conjugated target recognition reagent;
• and the parameter comprising a viral capsid protein marker signal, and/or a viral envelope marker signal, as well as a nucleic acid signal or a target nucleic acid signal;
• preferably, the parameter comprising a nucleic acid signal or a target nucleic acid signal and an envelope protein marker signal;
• step (2):
• detecting the sample solution comprising the viral nucleic acid vector by a flow particle analyzer, and recording data in a scattered channel and/or fluorescence channel;
• and
• step (3):
• analyzing the data and calculating results of the parameters,
• wherein the viral nucleic acid vector comprises a lentivirus vector, a retrovirus vector, a herpes simplex virus vector, and a recombinant coronavirus vector.