Method for determining basophil activation in a sample

Abstract

An in vitro method for determining basophil activation in a sample is provided. Other aspects relate to determining an efficacy of an allergen-specific desensitization treatment of a subject. The method includes providing a sample from the subject, treating the sample with an allergen and determining a level of at least one basophil inhibitory marker and at least one basophil activation marker in the sample, the basophil inhibitory marker includes CD32. Corresponding systems and machine learning methods are also described. The use of an affinity reagent that specifically binds CD32 in an in vitro method for determining basophil activation is also provided, for example in the context of determining an efficacy of an allergen-specific desensitization treatment of a subject. A kit and corresponding uses of affinity reagents is also detailed.

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