Composition, method and kit for pathology
OwnerRichard Rungeinventor
Life of this patent
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Abstract
Disclosed herein are embodiments of compositions, methods and kits that describe ancillary reagent constructs for simultaneous immunological and morphological investigation of pathology specimens. The kits include reagents, possibly organized in panels, which give the pathologist additional confidence in making a diagnosis based on the morphology of a cell. Reagents can be used or selected to identify specific antigens or a class of antigens. The reagents further comprise detection agents, which are visible under standard microscopy in the presence of standard stains. The reagents, used on their own or as part of a kit, make possible immunological analysis simultaneously with pathology performed in the presence of standard stains.
Claims8
-21. (canceled)
A method for conducting simultaneous immunological and morphological investigation comprising the steps of:
- isolating a specimen from a patient, the specimen including a hematology specimen or a pathology specimen;
- selecting at least one appropriate ancillary reagent construct with an antibody to an antigen expressed on a cell associated with a biological state of interest, the at least one appropriate ancillary reagent construct being visible at microscale under light microscopy and physically stable in the presence of an appropriate standard stain, wherein the at least one appropriate ancillary reagent construct comprises a detection agent, wherein the detection agent is a particle visible at microscale under light microscopy individually or in combination with additional ones of the detection agent;
- administering an appropriate amount of the at least one appropriate ancillary reagent construct;
- administering the appropriate standard stain;
- identifying, via light microscopy, stained cells with morphology consistent with the biological state of interest;
- and
- confirming, simultaneously with identification of the stained cells with morphology consistent with the biological state of interest, the biological state of interest based on the absence or presence of the detection agent proximal to the stained cells with morphology consistent with the biological state of interest and not proximal to the stained cells with morphology inconsistent with the biological state of interest.
Dependencies are inferred from the claim text.