US 2025/0052742 A1 · NOVEL FORMULATION FOR DRYING OF POLYMER DYE CONJUGATED ANTIBODIES

Abstract

A novel dry down buffer is provided for use in drying a plurality of fluorescent dye conjugates on a substrate for use in flow cytometry. The aqueous buffer comprises a water-soluble monomer; a protein stabilizer; a carbohydrate stabilizer; and a zwitterionic surfactant. When mixed with a multi-color panel comprising fluorescent polymer dye conjugates, dried on a substrate, and reconstituted with a biological sample, the buffer provides decreased aggregation of fluorescent polymer dye conjugates, and decreased non-specific binding of monocytes and granulocytes, when compared to use of a buffer without the water-soluble monomer or zwitterionic surfactant.

Claims31

Dependent claims

A buffer composition for use in drying a plurality of dye conjugates on a substrate, comprising

a water-soluble monomer comprising an aryl moiety or heteroaryl moiety;
a protein stabilizer;
a carbohydrate stabilizer;
and
a zwitterionic surfactant,
optionally wherein the composition is an aqueous composition, further optionally wherein the composition has a pH within a range of pH 6.5-7.5, or pH 7.0-7.4.

3 more elements

A composition comprising

one or more or a plurality of fluorescent polymer dye conjugates, optionally two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, 11 or more, 12 or more, or 2 to 20, 3 to 18, or 4 to 12 different dye conjugates each comprising a different binding partner;
a water-soluble monomer comprising an aryl moiety or heteroaryl moiety, optionally comprising one or more water-solubilizing moieties attached thereto;
a protein stabilizer;
a carbohydrate stabilizer;
and
a zwitterionic surfactant,
optionally wherein the composition is an aqueous composition, and further optionally wherein the composition has a pH within a range of pH 6.5-7.5, or pH 7.0-7.4.

4 more elements

Dependencies are inferred from the claim text.

Novel formulation for drying of polymer dye conjugated antibodies

Life of this patent

Novel formulation for drying of polymer dye conjugated antibodies

Life of this patent

Abstract

Claims31