Methods for Detecting a Protein in a Sample in a Fluidic Device Using Mass Spectrometry
AssigneeAmgen Inc.
InventorIain D.G. Campuzano
Life of this patent
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Abstract
The disclosure provides for methods of detecting and analyzing proteins using mass spectrometry analysis, such as matrix assisted laser desorption ionization mass spectrometry (MALDI-MIS) on protein directly within a fluidic device, or reversed phase liquid chromatographic mass spectrometry (rpLC-MS) on protein outside of the fluidic device.
Claims32
A method of detecting a protein in a sample, the method comprising subjecting the sample to mass spectrometry while the sample is in a fluidic device.
A method of detecting protein in samples in a fluidic device comprising:
- contacting samples in the device with solid supports, each solid support comprising a ligand for the protein, whereby the ligand binds to the protein of the sample, and wherein each solid support comprising the ligand bound to the protein comprises a unique barcode different from other solid supports comprising the ligand bound to the protein;
- transporting the solid support comprising the ligand bound to the protein from a first location in the fluidic device to a second location, wherein the second location is in the fluidic device or outside of the fluidic device;
- and
- subjecting the sample to mass spectrometry at the second location.
The method of any of the preceding claims, wherein the mass spectrometry is matrix-assisted laser desorption mass spectrometry/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF).
The method of any of the preceding claims, wherein the sample comprises conditioned media.
The method of any of the preceding claims, wherein the protein comprises or consists of a large peptide, antibody, antibody fragment, antibody fusion peptide or antigen-binding fragment thereof.
The method of any of the preceding claims, wherein the fluidic device is a microfluidic chip or sequestration pen.
The method of any one of the preceding claims, wherein the fluidic device comprises a silicon surface, and wherein the sample is disposed on the silicon surface.
A mass spectrometry plate comprising a fluidic device mounted to the surface of the mass spectrometry plate.
Dependencies are inferred from the claim text.