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US 2025/0052678 A1Published application
Evaluation method, evaluation device, and information processing program
AssigneeHamamatsu Photonics K.K.
InventorsKozo TAKEUCHI, Osamu YASUHIKO, Hidenao YAMADA
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Abstract
A refractive index distribution evaluation method, evaluation device, and information processing program, comprising: a data acquisition step or a data acquisition unit for acquiring refractive index distribution data for an object of observation; a selection step or a selection unit for selecting whether a target included in the refractive index distribution data is an evaluation target; and an evaluation step or an evaluation unit for evaluating the refractive index distribution of the evaluation target.
Claims10
1:
- A method for evaluating a refractive index distribution comprising:
- a data acquisition step of acquiring refractive index distribution data of an observation object;
- a selection step of selecting whether or not an object included in the refractive index distribution data is an evaluation object;
- and
- an evaluation step of evaluating the refractive index distribution of the evaluation object.
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2:
- The evaluation method according to claim 1, wherein
- the data acquisition step includes:
- acquiring for each of a plurality of light irradiation directions, an interference intensity image generated by interference between light irradiating the evaluation object along each of the plurality of light irradiation directions and passed through the evaluation object and reference light;
- a step of generating, for each of the plurality of light irradiation directions, a complex amplitude image at each of a plurality of positions based on the interference intensity image;
- a step of generating, for each of the plurality of positions, a complex differential interference image of each of the plurality of light irradiation directions based on the complex amplitude image of each of the plurality of light irradiation directions, and generating a two-dimensional phase image based on the complex differential interference image of each of the plurality of light irradiation directions;
- and
- a step of calculating a refractive index distribution based on the two-dimensional phase image at each of the plurality of positions.
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3:
- The evaluation method according to claim 1, wherein
- the selection step includes:
- a step of acquiring at least one refractive index cross sectional data in a certain direction from the refractive index distribution data;
- and
- a step of selecting whether or not an object included in the refractive index cross sectional data is an evaluation object.
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4:
- The evaluation method according to claim 1, wherein
- an evaluation method in the evaluation step includes:
- a step of extracting a region having a refractive index greater than or less than a threshold.
5:
- The evaluation method according to claim 4, wherein
- an evaluation method in the evaluation step includes:
- a step of dividing the region by its shape, its size, its density or its position in the observation object.
6:
- The evaluation method according to claim 4, wherein
- an evaluation method in the evaluation step includes:
- a step of specifying one or more positions in the evaluation object and dividing the evaluation object into a region present at a predetermined distance from the position and a region present at a distance farther than the predetermined distance from the position.
7:
- The evaluation method according to claim 1, wherein the observation object is a three-dimensional culture.
8:
- The evaluation method according to claim 1, wherein
- the refractive index distribution data includes:
- at least one refractive index data selected from the group consisting of a lipid droplet, a mitochondrion, a vesicle, a nucleolus and DNA.
9:
- An apparatus for evaluating a refractive index distribution comprising:
- a data acquisition unit for acquiring refractive index distribution data of an observation object;
- a selection unit for obtaining an indicator for an object included in the refractive index distribution data from the refractive index distribution data and selecting whether or not the object is an evaluation object based on the indicator;
- and
- an evaluation unit for evaluating the refractive index distribution of the evaluation object.
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10:
- An information processing program for causing a computer to execute steps:
- a data acquisition step of acquiring refractive index distribution data of an observation object;
- a selection step of obtaining an indicator for an object included in the refractive index distribution data from the refractive index distribution data and selecting whether or not the object is an evaluation object based on the indicator;
- and
- an evaluation step of evaluating the refractive index distribution of the evaluation object.
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