Attenuated Total Reflectance-Based Biosensor for Conformation and Secondary Structure Analysis
AssigneebetaSENSE GmbH
InventorsKlaus Gerwert, Jens Wiltfang, Julian Ollesch, Andreas Nabers, Jonas Schartner, Carsten Kötting
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Abstract
Provided herein is a biosensor for conformation and secondary structure analysis, notably for the direct non-invasive qualitative secondary structure analysis of a single selected protein within a complex mixture, as e.g. a body fluid, by vibrational spectroscopic methods. For the analysis it is not required that the selected substance be isolated, concentrated, or pretreated by a special preparative procedure.
Claims14
A method for determining the secondary structure of a candidate biomarker protein undergoing conformational transitions associated with disease progression in a body fluid, comprising the steps of:
- (a) conducting, in an infrared (IR) cell comprising an IR sensor element having at least one receptor capable of specific and conformationally independent binding to the candidate biomarker protein, a flux of body fluid comprising potential candidate biomarker proteins;
- (b) submitting an IR beam through said cell and obtaining an IR spectrum therefrom;
- and
- (c) analyzing the obtained IR spectrum comprising analyzing a shift of the amide I band maximum of the candidate biomarker protein to determine the secondary structure, and optionally the quantity, of the candidate biomarker protein present in the body fluid,
- wherein a downshift of the amide I band maximum of the candidate biomarker protein to a frequency below a threshold value of 1638-1648 cm−1 is indicative for disease progression.
Dependencies are inferred from the claim text.