Reference material for and method of calibrating raman spectrometers

A method of calibrating at least one Raman spectrometer, which comprises a monochromatic light source configured to transmit excitation light having an excitation wavelength to a measurement region configured to accommodate a sample of a medium as to illuminate the sample, and a spectrometric unit configured to receive measurement light emanating from the illuminated sample and configured to determine and provide intensity spectra of the received measurement light in a spectral measurement range, the method comprising:

• providing a reference material comprising quantum dots distributed in a transparent condensed phase material configured such that light emitted by the reference material in response to receiving light having the excitation wavelength has a predetermined spectral intensity distribution in the spectral measurement range;
• determining an emission spectrum of the reference material in response to the excitation wavelength;
• and
• calibrating each Raman spectrometer by:
• determining a reference spectrum of the reference material with the respective Raman spectrometer;
• and
• adjusting a determination of spectral intensity values of the intensity spectra performed by the respective Raman spectrometer based on the reference spectrum and the emission spectrum of the reference material.