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US 2025/0051966 A1Published application
Analysis of multiple analytes using a single assay
AssigneeIllumina, Inc.
InventorsFrank J. Steemers, Fan Zhang, Dmitry K. Pokholok, Steven Norberg
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Abstract
Embodiments of systems, methods, and compositions provided herein relate to methods of simultaneously analyzing multiple analytes in a single sample using a single assay. Some embodiments relate to simultaneous analysis of DNA and RNA in a single sample, for example, to the simultaneous generation of DNA and RNA libraries.
Claims20
A nucleic acid library comprising:
- a complementary DNA (cDNA) library derived from mRNA molecules and comprising nucleic acids having a 3′ polyA tail;
- and
- a genomic DNA (gDNA) library derived from genomic DNA and comprising nucleic acids having a 3′ polyA tail,
- wherein the cDNA and gDNA libraries are co-compartmentalized and prepared in the same environment.
1 more element
A flowcell device comprising:
- a polyT capture probe configured to hybridize to a 3′ polyA tail of RNA and a 3′ polyA tail that has been tagmented to DNA, and configured to simultaneously capture the DNA and RNA,
- wherein the polyT capture probe comprises a first common sequence (CS1),
- wherein the polyT capture probe is configured to confine the RNA and the DNA to a single compartment, thereby allowing simultaneous analysis of the RNA and the DNA from a sample, and
- wherein the RNA is converted to cDNA.
1 more element
A method of simultaneously analyzing in a single compartment DNA and RNA from a sample, comprising:
- providing a sample comprising DNA and RNA, wherein the RNA comprises a 3′ polyA tail;
- differentially tagging the DNA with a 3′ polyA tail;
- contacting the sample in a single compartment with a polyT capture probe for capturing the RNA and the tagged DNA, wherein the probe comprises a first common sequence (CS1);
- hybridizing the capture probe to the RNA and to the DNA, thereby simultaneously capturing DNA and RNA;
- converting the RNA to cDNA;
- and
- analyzing the cDNA and the DNA.
4 more elements
Dependencies are inferred from the claim text.