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AssigneeBoard of Regents, The University of Texas System
InventorsAndrew ELLINGTON, Simon D'OELSNITZ, Shaunak KAR, Ross THYER
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Disclosed herein are substrate-promiscuous regulators which have been engineered to function as highly efficient biosensors. These engineered biosensors are significantly more specific to the target ligand than their naturally occurring counterparts, and are able to generate a detectable output signal upon exposure to the input signal (target ligand). Also disclosed of methods of making engineered biosensors based on a naturally occurring substrate-promiscuous regulator. Also disclosed are methods of using these biosensors to make a product, such as cell-based bioengineering platforms. Lastly, disclosed are kits, nucleic acids, and proteins related to the biosensors disclosed herein.
A biosensor comprising an engineered substrate-promiscuous regulator, wherein said substrate-promiscuous regulator has been engineered to interact more efficiently with an input signal than does the naturally occurring substrate promiscuous regulator;
The plasmid of claim 19, wherein said plasmid further comprises a nucleic acid encoding the output signal.
A method of engineering a substrate-promiscuous regulator to function as a biosensor, the method comprising:
A kit comprising a biosensor comprising an engineered substrate-promiscuous regulator, wherein said substrate-promiscuous regulator has been engineered to interact more efficiently with an input signal than does the naturally occurring substrate promiscuous regulator.
A nucleic acid comprising 97% or more identity to any one of SEQ ID NOS:
Dependencies are inferred from the claim text.