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US 2025/0051856 A1Published application
DNA Methylation Analysis to Identify Cell Type
AssigneeInherent Biosciences, Inc.
InventorsTimothy JENKINS, Ryan BARNEY, Carter NORTON
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Abstract
Disclosed herein are methods of identifying a cell type by its genomic methylation pattern from a biological sample of a human subject. In some cases, the method can comprise identifying sperm and/or prostate cells. Also described herein are methods of identifying cell types for forensic analysis and for the treatment of a disease. The methods disclosed herein may comprise determining the DNA methylation pattern of DNA extracted from a semen sample. Also disclosed herein are methods of treating a disease or condition of a subject.
Claims20
A method for detecting a cancerous prostate cell comprising:
- a) obtaining a biological sample from a human subject, wherein the biological sample comprises seminal fluid that comprises a cell free DNA, a prostate cell comprising DNA, or both;
- b) extracting a DNA from a prostate cell in the biological sample or extracting a cell free DNA from the biological sample;
- c) detecting a methylation pattern of a region of the extracted DNA from the prostate cell in the biological sample or the extracted cell free DNA from the biological sample with a first computer program, executed on a computer, wherein the methylation pattern independently comprises a hypermethylation or a hypomethylation of at least one region of a chromosome, wherein the at least one region of the chromosome comprises:
- (i) a promoter of:
- ZFP64, MOB3B, or HLF or a portion of any of these;
- (ii) a gene comprising:
- TCAF-1, CHST-11, or ALOX-5 or a portion of any of these, or
- (iii) any combination of (i) and (ii);
- and
- d) comparing the methylation pattern of the at least one region of the extracted DNA from the prostate cell in the biological sample or the extracted cell free DNA from the biological sample to a control pattern of DNA methylation with a second computer program, executed on a computer thereby determining a presence or an absence of the cancerous prostate cell in the biological sample by the methylation pattern.
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Dependencies are inferred from the claim text.