Liquid biopsy assays for detecting nrg1 fusion polynucleotides
OwnerErnesto Isaac WASSERMAN, Jeroen Jilles LAMMERTS VAN BUERENinventors
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Abstract
This invention relates to liquid biopsy assays for the detection of neuregulin-1 (NRG1) fusion polynucleotides, particularly their use for diagnosing or treating disease, such as cancer. Novel neuregulin-1 (NRG1) fusion polynucleotides are also disclosed, as are kits for determining the presence or absence of an NRG1 fusion polynucleotide in a liquid biopsy sample. Methods of treating disease, such as cancer, are also disclosed.
Claims26
A method for determining the presence or absence of an NRG1 fusion polynucleotide in a sample, the method comprising:
- 1) providing a liquid biopsy sample obtained or derived from a subject having a solid tumor or a solid cancer, and
- 2) determining the presence or absence of the NRG1 fusion polynucleotide in the sample,
- wherein the NRG1 fusion polynucleotide is a fusion of an NRG1 polynucleotide with a second polynucleotide, which second polynucleotide does not encode NRG1, and wherein the NRG1 fusion polynucleotide is RNA.
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A method for determining the presence or absence of two or more NRG1 fusion polynucleotides in a sample, the method comprising:
- 1) providing a liquid biopsy sample, and
- 2) determining the presence or absence of the two or more NRG1 fusion polynucleotides in the sample, wherein the NRG1 fusion polynucleotides are RNA polynucleotides, and wherein the NRG fusion polynucleotides are fusions of an NRG1 polynucleotide with a second polynucleotide, which second polynucleotide does not encode NRG1.
A kit for determining the presence or absence of an NRG1 fusion polynucleotide in a liquid biopsy sample, comprising (a) a pair of forward and reverse polynucleotide primers which specifically hybridise to the NRG1 fusion polynucleotide and/or (b) a polynucleotide probe which specifically hybridises to the NRG1 fusion polynucleotide.
Dependencies are inferred from the claim text.