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US 2025/0051847 A1Published application
Analysis of nucleic acid sequences
Assignee10x Genomics, Inc.
InventorsSolongo B. ZIRALDO, Geoffrey McDermott, Shea Thompson Lance
Life of this patent
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Abstract
The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.
Claims30
A method comprising:
- (a) providing:
- a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and
- a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules;
- (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent;
- and
- (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.
3 more elements
A method comprising:
- (a) generating:
- a first emulsion comprising a first droplet population and micelles, wherein droplets of the first droplet population and the micelles comprise a chemical lysis agent;
- and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus comprising a target nucleic acid, and ii) a particle coupled to a plurality of nucleic acid barcode molecules;
- (b) contacting the first emulsion with the second emulsion, and subjecting the first emulsion and second emulsion to conditions sufficient to transfer the chemical lysis agent to the second droplet population via the micelles comprising the chemical lysis agent;
- and
- (c) lysing the cell or the nucleus within the droplet of the second droplet population with the chemical lysis agent, thereby releasing the target nucleic acid from the cell or the nucleus in the droplet of the second droplet population.
3 more elements
Dependencies are inferred from the claim text.