Method for measuring gene expression of single cell subpopulation, related kit, and application
AssigneesCytomics Limited, The Chinese University of Hong Kong
InventorsDan Huang, Kwong Sak Leung, Leung Sang Nelson Tang
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Abstract
The present application provides a peripheral blood sample analysis method and a use of a reagent component for measuring the abundance of a gene transcript in the preparation of a kit for the peripheral blood sample analysis method. The present application also provides a kit comprising the reagent component for quantifying the abundance of the gene transcript and a use of the reagent component for quantifying the abundance of the gene transcript in the preparation of a kit or drug for differentiating and triage of patients having abnormal body temperature.
Claims15
A method for analyzing a peripheral blood sample, comprising measuring, in the peripheral blood sample, the transcript abundance of a single cell subpopulation target gene and the transcript abundance of a single cell subpopulation reference gene, wherein the single cell subpopulation target gene is selected from at least one of those shown in Table 2-2, and the single cell subpopulation reference gene is selected from PSAP or CTSS, and preferably, the single cell subpopulation is monocytes.
Use of a reagent component for measuring the transcript abundance of genes in the preparation of a kit for use in a method for analyzing a peripheral blood sample, wherein the method comprises measuring, in the peripheral blood sample, the transcript abundance of a single cell subpopulation target gene and the transcript abundance of a single cell subpopulation reference gene, and wherein the single cell subpopulation target gene is selected from at least one of those shown in Table 2-2, and the single cell subpopulation reference gene is selected from PSAP or CTSS, and preferably, the single cell subpopulation is monocytes.
A kit, comprising a reagent component for quantifying the transcript abundance of genes, wherein the genes are selected from one or more of target genes shown in Table 2-2 and one or more of reference genes shown in Table 2-1.
Dependencies are inferred from the claim text.