Methods of labelling nucleic acids
AssigneeInivata Ltd.
InventorsSamuel Woodhouse, Tim Forshew, Stefanie Lensing
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Abstract
A method of sequencing a nucleic acid of interest (NAOI) is provided. In some embodiments, the method may comprise providing a sample from a patient, the sample having cell-free DNA molecules comprising NAOIs, amplifying the NAOIs, ligating adapters to the NAOIs, and sequencing the NAOIs. A method of labelling a NAOIs is also provided. In some embodiments, the method may comprise providing a sample from a patient having the NAOIs, amplifying the NAOIs, contacting the amplified NAOIs with a pool of oligonucleotides, attaching oligonucleotides to each of the amplified NAOIs to label the NAOIs. The method may further comprise sequencing the labelled NAOIs and grouping the resulting sequencing reads.
Claims20
A method of sequencing a Nucleic Acid of Interest (NAOI), the method comprising:
- (a) providing a sample from a patient, said sample comprising a plurality of cfDNA molecules, wherein the plurality of cfDNA molecules comprise a plurality of NAOIs;
- (b) amplifying the plurality of NAOIs;
- (c) ligating adapters to the plurality of NAOIs to produce adapter ligated NAOIs;
- and
- (d) sequencing the adapter ligated NAOIs.
A method of labelling a nucleic acid of interest (NAOI), the method comprising:
- (a) providing a sample from a patient, the sample comprising a plurality of cell-free DNA (cfDNA) molecules, wherein the cfDNA molecules comprise a plurality of NAOIs;
- (b) amplifying the plurality of NAOIs to produce amplicons;
- (c) contacting the amplicons with a pool of oligonucleotides;
- (d) attaching one or more oligonucleotides from the pool of oligonucleotides to one or both ends of each amplicon of the plurality of amplicons to provide a plurality of labelled NAOIs;
- (e) sequencing the labelled NAOIs to generate a library of sequencing reads;
- and
- (f) grouping the sequencing reads according to the sequences obtained from step (e).
Dependencies are inferred from the claim text.