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US 2025/0051837 A1Published application
Internal controls for nucleic acid amplification
AssigneeBecton, Dickinson and Company
InventorsGregory A. Richart, Sydney Bryce Tola, Ejan Marie Tyler, Honghua Zhang
Life of this patent
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Abstract
Disclosed herein include methods, kits, and reaction mixtures suitable for use in monitoring an amplification reaction. Some embodiments provide a first and a second quality control primer capable of hybridizing to each other and each comprising a quenchable label or a quencher.
Claims73
A method for monitoring an amplification reaction, comprising
- (a) providing a first quality control primer and a second quality control primer each comprising a 3′ overlapping region capable of hybridizing to each other, wherein each of the first quality control primer and the second quality control primer is no more than 35 nucleotides in length, and wherein the first quality control primer comprises a quenchable label;
- (b) contacting the first quality control primer and the second quality control primer, thereby forming a duplex via hybridization between the 3′ overlapping regions of the first and second quality control primers;
- (c) subjecting the duplex to an amplification condition, thereby generating an extended duplex;
- and
- (d) detecting a signal generated from the quenchable label of the first quality control primer during the amplification reaction to determine generation of the extended duplex, wherein a decrease in the signal during the amplification reaction indicates generation of the extended duplex.
2 more elements
A kit for monitoring an amplification reaction, comprising
- a first quality control primer and a second quality control primer each comprising a 3′ overlapping region capable of hybridizing to each other, wherein each of the first quality control primer and the second quality control primer is no more than 35 nucleotides in length, and wherein the first quality control primer comprises a quenchable label.
A reaction mixture, comprising
- a first quality control primer and a second quality control primer each comprising a 3′ overlapping region capable of hybridizing to each other, wherein each of the first quality control primer and the second quality control primer is no more than 35 nucleotides in length, and wherein the first quality control primer comprises a quenchable label;
- a target nucleic acid;
- and
- one or more additional primers and/or one or more probes specific to the target nucleic acid.
1 more element
Dependencies are inferred from the claim text.