Method for generating single strand and method for detecting mutation using same

A composition for detecting a target nucleic acid comprising:

• an isothermal one-pot reaction SENSR probe set for detecting a target nucleic acid including a first probe and a second probe;
• and
• at least one nucleic acid for interfering with double strand recombination of target gene,
• wherein the first probe is a promoter probe (PP) having a structure of the following general formula (I);
• 3′-X-Y-5′ (I)
• in the general formula (I) above,
• X is a stem-loop structure portion containing a promoter sequence recognizable by an RNA polymerase;
• Y is an upstream hybridization sequence (UHS) portion having a hybridization sequence complementary to a target nucleic acid sequence;
• the target nucleic acid sequence is DNA or RNA;
• and X and Y are deoxyribonucleotides;
• the second probe is a reporter probe (RP) having a structure of the following general formula II;
• 3′-Y′-Z-5′ (II)
• in the general formula (II) above,
• Y′ is a downstream hybridization sequence (DHS) portion having a hybridization sequence complementary to the target nucleic acid sequence;
• Z is an aptamer sequence portion with an interactive labeling system comprising one label or a plurality of labels generating a detectable signal;
• the target nucleic acid sequence is DNA or RNA;
• and Y′ and Z are deoxyribonucleotides;
• and
• the nucleic acid for interfering with double strand recombination of target gene is a sequence complementary to the target nucleic acid sequence at the 3′ end or 5′ end of a complementary binding region of the SENSR probe set.