Ex Vivo Production of Compounds of Interest
AssigneeNederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
InventorsEvita VAN DE STEEG, Wouter Henricus Johannes VAES, Lida Joanne STEVENS, Steven Leonardus Angela ERPELINCK
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Abstract
The invention relates to methods for producing one or more compounds of interest, comprising ex vivo perfusing a liver or liver tissue and optionally a kidney or kidney tissue with a perfusion liquid comprising a precursor of said one or more compounds of interest, wherein said precursor is a compound that is metabolized in the liver and/or kidney, and purifying said one or more compounds of interest from the perfusate, bile, urine, perfused liver or liver tissue and/or perfused kidney or kidney tissue.
Claims20
A method for producing one or more compounds of interest, the method comprising:
- ex vivo perfusing a liver or liver tissue and optionally a kidney or kidney tissue with a perfusion liquid comprising at least 50 mg of a precursor of said one or more compounds of interest, wherein said precursor is a compound that is metabolized in the liver and/or kidney, and
- purifying said one or more compounds of interest from the perfusate, bile, urine, perfused liver or liver tissue and/or perfused kidney or kidney tissue
- wherein said liver or liver tissue and optionally kidney or kidney tissue is obtained from a slaughterhouse,
- wherein the weight of the liver or liver tissue is at least about 250 gr.,
- wherein the perfusion liquid is continuously oxygenated, and
- wherein the liver or liver tissue comprises a portal vein and a hepatic artery and the liver or liver tissue is perfused through both the portal vein and the hepatic artery.
Dependencies are inferred from the claim text.