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US 2025/0051823 A1Published application
Products and methods for heterologous expression of proteins in a host cell
AssigneeAbSci LLC
InventorsMiles Gander, Amy E. Doerner, Carla Risso, Gregory Hannum, Ariel Schwartz
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Abstract
The disclosure provides products and methods for the heterologous expression of proteins in host cells. The provided expression constructs, bacterial cells and methods produce a PnlP-1 accessory protein that increases the yield of a heterologous protein of interest.
Claims20
An expression construct comprising:
- a) a polynucleotide encoding an accessory protein of SEQ ID NO:
- 2,
- b) a polynucleotide encoding an accessory protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:
- 2, or
- c) a polynucleotide encoding an accessory protein of SEQ ID NO:
- 2 or encoding an accessory protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:
- 2, and a polynucleotide encoding a protein of interest.
4 more elements
A host cell comprising:
- a) an expression construct according to any preceding claim, or
- b) an expression construct according to a preceding claim which does not include a polynucleotide encoding a protein of interest and a second expression construct comprising a polynucleotide encoding a protein of interest.
Dependencies are inferred from the claim text.