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AssigneeBASF SE
InventorsMax Fabian Felle, Christopher Sauer, Mathis Appelbaum, Stefan Jenewein, Sebastian Schoof, Christian Riedele
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The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.
A method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of
A Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.
A method of producing a Bacillus host cell comprising
Dependencies are inferred from the claim text.