Production of rebaudioside
AssigneeSamyang Corporation
InventorsHyeokjin KO, Junho MOON, Jeongmin KIM, Min Young KIM, Busoo PARK
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a composition for producing steviol glycosides containing Rebaudioside M at high purity, using a biocatalyst containing UDP-glycosyltransferase and UGT76G1, and a method for producing steviol glycosides using the same.
Claims25
A composition for producing steviol glycosides containing Rebaudioside M,
- which comprises a biocatalyst comprising a first uridine diphosphate-glucosyltransferase (UDP-glucosyltransferase) that has 92% or higher of amino acid sequence identity with an amino acid sequence of SEQ ID NO:
- 1 and transfers glucose to steviol glycoside substrate, and UGT76G1;
- and a reaction substrate;
- wherein Rebaudioside M is contained in 10% by weight or more of the steviol glycosides reaction product.
4-6. (canceled)
9-10. (canceled)
(canceled)
19-20. (canceled)
A method for producing steviol glycosides containing Rebaudioside M, comprising reacting a reaction substrate containing at least one selected from the group consisting of stevioside and Rebaudioside A, with a biocatalyst containing a first uridine diphosphate-glucosyltransferase and UGT76G1,
- wherein the first uridine diphosphate-glucosyltransferase has an amino acid sequence identity of 92% or higher with an amino acid sequence of SEQ ID NO:
- 1, and has UDP-glycosyltransferase activity transferring glucose to a steviol glycoside substrate, and
- wherein the Rebaudioside M is contained at 10% by weight or more of the steviol glycosides reaction product.
(canceled)
Dependencies are inferred from the claim text.