Metabolically engineered organisms for the production of added value bio-products
AssigneeInbiose N.V.
InventorsJo Maertens, Joeri Beauprez, Marjan De Mey
Life of this patent
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Abstract
The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.
Claims22
1-29. (canceled)
A metabolically engineered bacterium or yeast for the production of a specialty product selected from the group consisting of a carbohydrate specialty product, an O-glycoside or a glycolipid, characterized in that said bacterium or yeast:
- a) has been genetically modified by introducing a heterologous gene encoding a sucrose synthase capable of splitting sucrose into UDP-glucose and fructose;
- and
- b) has been further genetically modified to prevent loss of UDP-glucose via glycolysis due to the genetic disruption of an endogenous gene encoding an UDP-glucose hydrolase, an UDP-glucose-4-epimerase, a glucose-1-phosphate uridilyltransferase, an UDP-glucose 6-dehydrogenase, an UDP-glucose diphosphorylase, an UDP-glucose-hexose-1-phosphate uridyltransferase, an UDP-glucose-glycogen glucosyltransferase, an UDP-glucose-1,3-beta-D-glucan glucosyltransferase, an UDP-glucose-glucosephosphate glucosyltransferase, or a combination thereof.
Dependencies are inferred from the claim text.