Methods
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsMichael Anthony AYLIFFE, Thomas David Loan, Ming Luo
Life of this patent
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Abstract
The present invention relates to methods of increasing bromoform production.
Claims32
A method of producing bromoform, the method comprising incubating an organism or part thereof, cells, a lysate of the organism or part thereof or cells, or a mixture thereof, or conditioned medium obtained from culturing a microorganism, cells, a lysate of the microorganism or cells, comprising a vanadate-dependent haloperoxidase, in the presence of at least one compound of Formula 1:
- wherein R1 and R2 are:
- independently selected from:
- hydrogen, hydroxyl, optionally substituted aliphatic, optionally substituted O-alkyl, or optionally substituted S-alkyl, or
- R1 and R2 are joined to form an optionally substituted six membered ring of Formula 1a:
- wherein R3, R4, R5, R6, R7, and R8 are each independently selected from hydrogen or an optionally substituted aliphatic.
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A method of producing bromoform, the method comprising incubating a vanadate-dependent haloperoxidase in the presence of at least one compound of Formula 1:
- wherein R1 and R2 are:
- independently selected from:
- hydrogen, hydroxyl, optionally substituted aliphatic, optionally substituted O-alkyl, or optionally substituted S-alkyl, or
- R1 and R2 are joined to form an optionally substituted six membered ring of Formula 1a:
- wherein R3, R4, R5, R6, R7, and R8 are each independently selected from hydrogen or an optionally substituted aliphatic, and wherein one or more or all of the following apply:
- i) the vanadate-dependent haloperoxidase is not present in a protein extract obtained from an organism which naturally produces the vanadate-dependent haloperoxidase,
- ii) the vanadate-dependent haloperoxidase is incubated with the at least one compound for greater than 90 minutes,
- iii) the incubating occurs in the presence of hydrogen peroxide, and if hydrogen peroxide is added more than once, then at least two of the additions are greater than 10 minutes apart,
- iv) the compound is not oxaloacetic acid nor acetylacetone.
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Dependencies are inferred from the claim text.