Engineered viral particles and uses of the same
AssigneeInterius Biotherapeutics, Inc.
InventorsJames I. Andorko, Mohit Sachdeva
Life of this patent
Estimating the term…
Abstract
Provided for herein are compositions and methods that can be used to transduce cells and deliver genetic information that can then be expressed in the transduced cell. Also provided herein are methods of treating a disease in a subject using the compositions and methods provided.
Claims108
An pseudotyped viral-like particle or viral vector comprising:
- a targeting polypeptide comprising a heterologous targeting moiety linked to a protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family;
- a truncated protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family, wherein the truncated protein does not comprise a targeting moiety;
- a glycoprotein derived from an envelope glycoprotein F of a virus of the Paramyxoviridae family,
- wherein the targeting polypeptide and the truncated protein comprise the same or different envelope glycoproteins.
A pseudotyped viral-like particle or viral vector comprises a plurality of polypeptides comprising the general amino acid formula of:
- [PG]-[RR]-Linker-[TM];
- [EG];
- and
- [GF], wherein
- PG is an envelope glycoprotein G or H sequence of a virus of the Paramyxoviridae family;
- RR is an optional restriction site sequence;
- Linker is a peptide linker;
- TM is a targeting moiety;
- EG is an envelope glycoprotein G or H of a virus of the Paramyxoviridae family;
- and
- GF is an envelope glycoprotein F sequence of a virus of the Paramyxoviridae family.
The method of embodiment 100, wherein the target cell is an immune cell, T cell, B cell, NK cell, dendritic cell, neutrophils, macrophages, cancer cell, CD3+ T cell, CD4+ T cell, CD7+ T cell, CD8+ T cell, CD19+ B cell, CD19+ cancer cell, CD20+ B cell, CD30+ lung epithelial cell, CD34+ haematopoietic stem cell, CD105+ endothelial cell, CD105+ haematopoietic stem cell, CD117+ haematopoietic stem cell, CD133+ cancer cell, EpCAM+ cancer cell, GluA2+ neuron, GluA4+ neuron, Haematopoietic stem cell, Hepatocyte, Her2/Neu+ cancer cell, NKG2D+ natural killer cell, SLC1A3+ astrocyte, and SLC7A10+ adipocyte.
A nucleic acid molecule encoding a targeting polypeptide comprising a heterologous targeting moiety linked to a protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family;
- a truncated protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family, wherein the truncated protein does not comprise a targeting moiety;
- a glycoprotein derived from an envelope glycoprotein F of a virus of the Paramyxoviridae family,
- wherein the targeting polypeptide and the truncated protein comprise the same or different envelope glycoproteins.
A plurality of nucleic acid molecules encoding a targeting polypeptide comprising a heterologous targeting moiety linked to a protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family;
- a truncated protein derived from an envelope glycoprotein G or H of a virus of the Paramyxoviridae family, wherein the truncated protein does not comprise a targeting moiety;
- a glycoprotein derived from an envelope glycoprotein F of a virus of the Paramyxoviridae family,
- wherein the targeting polypeptide and the truncated protein comprise the same or different envelope glycoproteins.
Dependencies are inferred from the claim text.