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US 2025/0051794 A1Published application
Tobacco plants having increased nitrogen efficiency and methods of using such plants
AssigneeAltria Client Services LLC
InventorsJesse Frederick, Chengalrayan Kudithipudi, Dongmei Xu
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Abstract
This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.
Claims21
1-18. (Canceled)
A tobacco plant comprising an induced mutation in a MADS-box transcription factor (TF) 27-like gene, wherein the MADS-box TF 27-like gene comprises an allele comprising a nucleic acid sequence of SEQ ID NO:
- 13 or SEQ ID NO:
- 14, wherein the induced mutation encodes an introduced stop codon that results in a truncated MADS-box TF 27-like polypeptide, and wherein the induced mutation causes a loss-of-function of the truncated MADS-box TF 27-like polypeptide.
A method for producing a tobacco plant, the method comprising:
- (a) inducing mutagenesis in tobacco cells to produce mutagenized cells;
- (b) obtaining one or more tobacco plants from the mutagenized cells;
- and
- (c) identifying at least one tobacco plant obtained in step (b) that comprises an induced mutation an endogenous nucleic acid encoding a MADS-box transcription factor (TF) 27-like polypeptide, wherein the endogenous nucleic acid comprises the nucleic acid sequence of SEQ ID NO. 13 or SEQ ID NO:
- 14, wherein the induced mutation comprises an introduced stop codon that results in a truncated a MADS-box TF 27-like polypeptide, and wherein the induced mutation causes a loss-of-function of the MADS-box TF 27-like gene polypeptide.
2 more elements
Dependencies are inferred from the claim text.