Improved screening method for genome edited events

A method for the production of a plant comprising a desired nucleic acid sequence comprising the steps:

• (a1) (i) providing a population of plant cells comprising regenerative plant cells expected to have a desired nucleic acid sequence, (ii) dividing the population of the plant cells comprising regenerative plant cells into subgroups, (iii) extracting DNA from one or more samples from said cells of subgroups, (iv) identifying one or more subgroups with the highest concentration(s) of the desired nucleic acid sequence, and (v) selecting one or more subgroups of said cells with the highest concentration of the desired nucleic acid sequence,
• whereby, optionally, cells are genetically modified cells,
• or
• (a2) (i) dividing the population plant cells comprising regenerative plant cells into subgroups, (ii) optionally, genetically modifying the subgroups of plant cells comprising regenerative plant cells, (iii) extracting DNA from one or more samples from said cells of each subgroup, (iv) identifying one or more subgroups with the highest concentration(s) of a desired nucleic acid sequence, and (v) selecting one or more subgroups of said cells with the highest concentration(s) of the desired nucleic acid sequence,
• and
• (b) (i) culturing the regenerative plant cells of the selected subgroup(s) of step (a1) or (a2), (ii) extracting DNA from one or more samples from the cultured plant cells of the subgroups, (iv) identifying one or more subgroups with the highest concentration(s) of the desired nucleic acid sequence, and (v) selecting one or more subgroups of said cultured cells with the highest concentration(s) of the desired nucleic acid sequence,
• and,
• (c1) (i) recovering individual plants or shoots from the cells of the selected subgroup(s) from step (b), (ii) dividing the population of plants or shoots into subgroups, (ii) extracting DNA and/or RNA from one or more samples from cells of each subgroup, (iii) identifying one or more subgroups with the highest concentration(s) of the desired nucleic acid sequence, and (iv) selecting one or more subgroups of said plants or shoots with the highest concentration(s) of the desired nucleic acid sequence and grow plants thereof,
• or
• (c2), (i) recovering individual plants or shoots from the cultured cells of the selected subgroup(s) from step (b), (ii) taking DNA and/or RNA comprising samples from each plant or shoot, (iii) analyzing said DNA and/or RNA for presence of the desired nucleic acid sequence, (iv) selecting the plants with the desired nucleic acid sequence and grow plants thereof,
• whereby, optionally, the plant cells are genetically modified cells.